Micro 20 Exam 2 Review


Can you describe the 4 biosafety levels in your own words? [essay]

Review the text to compare/contrast direct and indirect methods of quantifying bacterial growth [essay]

Describe the compliment system and list three results of compliment activation [essay]

List three disinfectants and describe how they function against microbes [essay]

Know the general processes of glycolysis, cell respiration, and fermentation.  Understand electron transfer and the role it plays in aerobic respiration.  Be able to identify the components of the electron transfer system, functioning to produce an H+ gradient that powers ATP production.

Know the differences, in general, between photoheterotrophs, photoautotrophs, chemoheterotrophs, and chemoautotrophs.

Know the general growth requirements and how changes in specific parameters might affect bacterial growth rates.  Think in terms of how the rate might be graphed as a result of different parameters, such as pH, temperature, availability of various chemicals, presence of oxygen, etc.

What are the differences between facultative, tolerant, and obligate microbes (i.e., facultative anaerobe vs. obligate anaerobe?) REVIEW the figure in the text.

Be able to graph microbial growth and death, and know the different phases related to the graph.

What are biofilms and what is the significance of biofilms?

Be able to address the different types of media (complex, chemical, differential, selective, enrichment, etc.)

Know the factors that influence microbial death, including the main actions of the control agents.  What is TDP, DRT, TDT.? Be able to use graphic information to infer details regarding TDP, DRT, and TDT.

Know how heat sterilization works, what Pasteurization is, and when filtration is appropriate.

Be aware of the various physical methods of controlling microbial growth, including radiation approaches.

Know how to measure the effectiveness of a microbial growth control approach (e.g. use dilution test).

Understand the basics of immunity:

Know the first line of defense, including physical/chemical factors and normal microbiota.

Know the second line of defense, including formed blood elements (and what they do), the lymph system, phagocytotic process, inflammatory and fever response process (think about the steps involved), and generalities of the compliment system.  Be able to state what 'margination' is, what is found in blood serum, the function of the ciliary escalator, etc.